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Table 1 Effect of metal ions, chelators, and detergents on recombinant VxAly7D activity

From: Biochemical characteristics and molecular mechanism of an exo-type alginate lyase VxAly7D and its use for the preparation of unsaturated monosaccharides

Reagents added Concentration Relative activity (%)
None 0 mM 100.00 ± 2.03
LiCl 1 mM 147.17 ± 2.83
NH4Cl 1 mM 156.35 ± 5.23
MgCl2 1 mM 108.27 ± 2.50
CaCl2 1 mM 215.14 ± 6.92
MnCl2 1 mM 145.73 ± 4.01
FeSO4 1 mM 144.68 ± 3.10
NiCl2 1 mM 65.03 ± 0.98
CuSO4 1 mM 6.48 ± 2.04
ZnCl2 1 mM 25.13 ± 3.10
BaCl2 1 mM 130.19 ± 5.28
FeCl3 1 mM 61.03 ± 1.29
EDTA 1 mM 21.20 ± 1.05
EGTA 1 mM 49.55 ± 1.67
DTT 1 mM 71.77 ± 1.03
SDS 3% 2.40 ± 0.23