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Table 1 Effect of metal ions, chelators, and detergents on recombinant VxAly7D activity

From: Biochemical characteristics and molecular mechanism of an exo-type alginate lyase VxAly7D and its use for the preparation of unsaturated monosaccharides

Reagents added

Concentration

Relative activity (%)

None

0 mM

100.00 ± 2.03

LiCl

1 mM

147.17 ± 2.83

NH4Cl

1 mM

156.35 ± 5.23

MgCl2

1 mM

108.27 ± 2.50

CaCl2

1 mM

215.14 ± 6.92

MnCl2

1 mM

145.73 ± 4.01

FeSO4

1 mM

144.68 ± 3.10

NiCl2

1 mM

65.03 ± 0.98

CuSO4

1 mM

6.48 ± 2.04

ZnCl2

1 mM

25.13 ± 3.10

BaCl2

1 mM

130.19 ± 5.28

FeCl3

1 mM

61.03 ± 1.29

EDTA

1 mM

21.20 ± 1.05

EGTA

1 mM

49.55 ± 1.67

DTT

1 mM

71.77 ± 1.03

SDS

3%

2.40 ± 0.23

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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