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Table 2 Comparison of biochemical characterization of recombinant VxAly7D and AlyD

From: Biochemical characteristics and molecular mechanism of an exo-type alginate lyase VxAly7D and its use for the preparation of unsaturated monosaccharides

Parameter VxAly7D AlyD
Origin V. xiamenensis QY104 V. splendidus 12B01
Molecular mass 36.46 kDa 36.5 kDa
Optimal pH 7.3 8
Optimal temperature 30 °C 20 °C
Optimal NaCl concentration 100 mM 400 mM
Metal ions
 Inhibition Cu2+, Ni2+, Zn2+ Cu2+, Ni2+, Zn2+, Fe2+, Mg2+, Mn2+
 Promotion Ca2+, Fe2+, Mg2+, Mn2+ Ca2+
EDTA Inhibit Inhibit
Substrate preference Sodium alginate (462.40 U/mg) Alginate (0.5 μM/s/μg)
polyG (357.37 U/mg) polyG (0.41 μM/s μg)
polyM (441.94 U/mg) polyM (0.25 μM/s μg)
Products Mono-, di-, trisaccharides Mono-, di-, pentasaccharides
Action mode Exo-type Endo-type
Reference In this study [46]