Table 2 Comparison of biochemical characterization of recombinant VxAly7D and AlyD

Origin

V. xiamenensis QY104

V. splendidus 12B01

Molecular mass

36.46 kDa

36.5 kDa

Optimal pH

7.3

8

Optimal temperature

30 °C

20 °C

Optimal NaCl concentration

100 mM

400 mM

Metal ions

 Inhibition

Cu²⁺, Ni²⁺, Zn²⁺

Cu²⁺, Ni²⁺, Zn²⁺, Fe²⁺, Mg²⁺, Mn²⁺

 Promotion

Ca²⁺, Fe²⁺, Mg²⁺, Mn²⁺

Ca²⁺

EDTA

Inhibit

Inhibit

Substrate preference

Sodium alginate (462.40 U/mg)

Alginate (0.5 μM/s/μg)

polyG (357.37 U/mg)

polyG (0.41 μM/s μg)

polyM (441.94 U/mg)

polyM (0.25 μM/s μg)

Products

Mono-, di-, trisaccharides

Mono-, di-, pentasaccharides

Action mode

Exo-type

Endo-type

Reference

In this study

[46]