Fig. 5
From: Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases
The key residues associated with alkaline adaptability. a CrmE10 (top, PDB: 7C23) and AlinE4 (bottom, PDB: 7C82) are indicated in electrostatic potential surfaces. Red: negative potential; blue: positive potential. Five residues associated with of alkaline adaptability are indicated in sticks (green: CrmE10, blue: AlinE4). Enzymatic activities of CrmE10 (b) and AlinE4 (c) and their mutants determined with a range of different pH values. The value obtained at pH 7.5 was taken as 100%. The gap was between different pH due to the buffer changing