Fig. 2
From: A novel C-terminal degron identified in bacterial aldehyde decarbonylases using directed evolution
Discovery of a degron in ADpm via directed evolution. a Schematic of directed evolution system designed for AD evolution. b Distribution analysis of the mutations across the α-helices of ADpm. c Crystal structure of ADpm (PDBID: 2OC5). There are 8 α-helices in AD structure. α-helix 1, 2, 4 and 5 form the catalytic domain. d GFP degradation test with and without the ADpmC1−9 sequence. Fluorescence values were normalized to the number of cells by dividing by the OD600. Data show the mean value of five replicates