Skip to main content
Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: A novel C-terminal degron identified in bacterial aldehyde decarbonylases using directed evolution

Fig. 5

Investigation of degradation mechanism of AD degron. a Effects of protease deletion on GFP degradation with data normalized by OD600. b Diagram illustrating the recognition and degradation process of AD degron-tagged protein. GFP-A, GFP-B, GFP-C and GFP-ADcon refer to Fig. 4d. AD degron can be recognized by the ClpA and Lon protease complexes. ClpA-bound GFP will be unfolded and translocated into ClpP for subsequent degradation. Lon-bound GFP will be unfolded and degraded simultaneously in itself. Fluorescence values were normalized to the number of cells by dividing by the OD600. The data shown are from the mean of three biological replicates

Back to article page