Fig. 6
From: A novel C-terminal degron identified in bacterial aldehyde decarbonylases using directed evolution
Investigation of degron modification. Representative ADs from P. marinus (ADpm), N. punctiforme (ADnp) and S. elongatus (ADse). a-c Comparison of specific activities, enzyme abundance and pentane production in three representative ADs with and without degron. Enzyme abundance was normalized to ADpm based on peak area. d Comparison of enzyme abundance and pentane production in ADse C-terminal His-tag and N-terminal His-tag. e Modeling of protein–protein interaction between ADse and ferredoxin from E. coli. E. coli 2Fe2S ferredoxin (Uniprot ID P0A9R4) (colored pink) and ADse decarbonylase (colored wheat) interaction model predicted with GRAMMX protein–protein docking server. The C- and N-terminal ends of ADse are designated. f C-terminal His-tag effect on degron sealing. The data shows the mean of three replicates, and all error bars show the S.D