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Table 2 Substrate specificity of CmNAGase

From: A novel bacterial β-N-acetyl glucosaminidase from Chitinolyticbacter meiyuanensis possessing transglycosylation and reverse hydrolysis activities

Substratesa

Specific activity (U/mg of protein)

Colloidal chitin

0.02 ± 0.001

pNP-GlcNAc

4878.6 ± 200.8

pNP-Glucoside

–

pNP-Acetylgalactosaminide

–

4-MU-GlcNAc

4206.1 ± 283.8

Cellobiose

–

(GlcNAc)2

5305.4 ± 125.6

(GlcNAc)3

3132.8 ± 280.5

(GlcNAc)4

1409.4 ± 67.3

(GlcNAc)5

1116.2 ± 45.1

(GlcNAc)6

473.7 ± 36.8

  1. –: Activity was not detected
  2. aReaction mixture (1 mL) containing enzyme (3 μg) and substrates (10 g/L) was incubated at pH 5.4 and 40 °C for 10 min
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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