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Table 2 Substrate specificity of CmNAGase

From: A novel bacterial β-N-acetyl glucosaminidase from Chitinolyticbacter meiyuanensis possessing transglycosylation and reverse hydrolysis activities

Substratesa Specific activity (U/mg of protein)
Colloidal chitin 0.02 ± 0.001
pNP-GlcNAc 4878.6 ± 200.8
pNP-Glucoside
pNP-Acetylgalactosaminide
4-MU-GlcNAc 4206.1 ± 283.8
Cellobiose
(GlcNAc)2 5305.4 ± 125.6
(GlcNAc)3 3132.8 ± 280.5
(GlcNAc)4 1409.4 ± 67.3
(GlcNAc)5 1116.2 ± 45.1
(GlcNAc)6 473.7 ± 36.8
  1. –: Activity was not detected
  2. aReaction mixture (1 mL) containing enzyme (3 μg) and substrates (10 g/L) was incubated at pH 5.4 and 40 °C for 10 min