TY - JOUR AU - Wu, Wenyang AU - Du, Wei AU - Gallego, Ruth Perez AU - Hellingwerf, Klaas J. AU - van der Woude, Aniek D. AU - Branco dos Santos, Filipe PY - 2020 DA - 2020/07/02 TI - Using osmotic stress to stabilize mannitol production in Synechocystis sp. PCC6803 JO - Biotechnology for Biofuels SP - 117 VL - 13 IS - 1 AB - Mannitol is a C(6) polyol that is used in the food and medical sector as a sweetener and antioxidant, respectively. The sustainable production of mannitol, especially via the direct conversion of CO2 by photosynthetic cyanobacteria, has become increasingly appealing. However, previous work aiming to achieve mannitol production in the marine Synechococcus sp. PCC7002 via heterologous expression of mannitol-1-phosphate-5-dehydrogenase (mtlD) and mannitol-1-phosphatase (m1p, in short: a ‘mannitol cassette’), proved to be genetically unstable. In this study, we aim to overcome this genetic instability by conceiving a strategy to stabilize mannitol production using Synechocystis sp. PCC6803 as a model cyanobacterium. SN - 1754-6834 UR - https://doi.org/10.1186/s13068-020-01755-3 DO - 10.1186/s13068-020-01755-3 ID - Wu2020 ER -