Fig. 1From: Using osmotic stress to stabilize mannitol production in Synechocystis sp. PCC6803Overview of the engineered biosynthetic pathway to mannitol in the cyanobacterium Synechocystis sp. PCC6803. Solid arrows without wings represent native steps in cell; dashed arrows represent introduced pathway of mannitol synthesis; engineered enzymes are shown in blue; red circles with diagonal red line indicate deleted proteins; red cross-lines indicate metabolites that cannot be synthesized. White double circles represent protection against osmotic pressure by compatible solutes or mannitol in the cells. Grey ovals represent salt stress. 3PG, 3-phosphoglycerate; F6P, fructose-6-phosphate; G3P, glyceraldehyde-3-phosphate; M1P, mannitol-1-phosphate; M1p, Mannitol-1-phosphatase (M1Pase; encoded by m1p from E.tenella); MtlD, Mannitol-1-phosphate dehydrogenase (M1PDH; encoded by mtlD from E. coli); GgpS, Glucosyl-glycerol phosphate synthase; GG-P, glucosyl-glycerol-phosphate; GgpP, Glucosylglycerol phosphate phosphatase; GG, glucosyl-glycerol; Sps, Sucrose phosphate synthase; S6P, sucrose-6-phosphate; Spp, Sucrose phosphate phosphatase; RuBP, ribulose-1,5-bisphosphateBack to article page