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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Using osmotic stress to stabilize mannitol production in Synechocystis sp. PCC6803

Fig. 1

Overview of the engineered biosynthetic pathway to mannitol in the cyanobacterium Synechocystis sp. PCC6803. Solid arrows without wings represent native steps in cell; dashed arrows represent introduced pathway of mannitol synthesis; engineered enzymes are shown in blue; red circles with diagonal red line indicate deleted proteins; red cross-lines indicate metabolites that cannot be synthesized. White double circles represent protection against osmotic pressure by compatible solutes or mannitol in the cells. Grey ovals represent salt stress. 3PG, 3-phosphoglycerate; F6P, fructose-6-phosphate; G3P, glyceraldehyde-3-phosphate; M1P, mannitol-1-phosphate; M1p, Mannitol-1-phosphatase (M1Pase; encoded by m1p from E.tenella); MtlD, Mannitol-1-phosphate dehydrogenase (M1PDH; encoded by mtlD from E. coli); GgpS, Glucosyl-glycerol phosphate synthase; GG-P, glucosyl-glycerol-phosphate; GgpP, Glucosylglycerol phosphate phosphatase; GG, glucosyl-glycerol; Sps, Sucrose phosphate synthase; S6P, sucrose-6-phosphate; Spp, Sucrose phosphate phosphatase; RuBP, ribulose-1,5-bisphosphate

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