Fig. 5
Impact of nitrogen deprivation on cellular compartment abundance in M. alpina. Venn diagram shows overlap in numbers of metabolites (a) and proteins (b) that were significantly upregulated or downregulated. c–f Volcano plots show changes in proteins assigned by gene ontology to specific compartments at 96 h vs. 24 h (Log 2 FC > 2, p < 0.05). Protein abundance was determined using tandem mass spectrometry and quantified by MS1 precursor ion intensity. Protein annotations are described in detail in Additional file 5