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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Metabolic engineering of phosphite metabolism in Synechococcus elongatus PCC 7942 as an effective measure to control biological contaminants in outdoor raceway ponds

Fig. 2

Growth competition experiments between SeptxD-2 and Chlorella sorokiniana using phosphite as the only phosphorus source. aS. elongatus transgenic strain SeptxD-2 and Chlorella sorokiniana UTEX 1230 (CsWT) were grown as monocultures in BG-11 media with phosphate (Pi, 0.2 mM) as the phosphorus (P) source, and as monocultures and mixed cultures in three different inocula proportions SeptxD-2:CsWT (1:1, 1:4, and 4:1) in BG-11 media with 1.8 mM phosphite (Phi) as P source. Cultures were photographed 8 days after inoculation. Cell number (cells/mL) was determined for the experiments with different proportions, b 1:1, c 1:4, d 4:1, every 2 days. e Cultures on (a, b) were analyzed by light microscopy 8 days after inoculation. Black bar corresponds to 20 μm. Values are the mean of three replicates ± SD. Bars with asterisks are significantly different from the control (Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.0001)

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