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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Metabolic engineering of phosphite metabolism in Synechococcus elongatus PCC 7942 as an effective measure to control biological contaminants in outdoor raceway ponds

Fig. 4

SeptxD-2 cultivation in outdoor cylindrical reactors using phosphite as phosphorus source under non-sterile conditions. aS. elongatus PCC 7942 (SeWT) and SeptxD-2 transgenic strain were grown in BG-11 media prepared with industrial grade reagents and supplemented with 1.8 mM phosphite (Phi) and 0.2 mM phosphate (Pi) as P source, respectively. b Cell number (cells/mL) and c biomass were determined every 2 days during the experiments. d Colony Forming Units (CFU/mL) derived from SeptxD-2 and SeWT cultures. Cultures were performed using 7% (v/v) inoculum and non-sterile 100-L cylindrical reactors bubbled with air outdoor. Values are the mean ± SD of three replicates. Bars with asterisks are significantly different from the control (Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.0001). Photographs correspond to experiments carried out during summer, 2017

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