Fig. 2

Subcellular localization of PtPTP in P. tricornutum.a Membrane topology of PtPTP predicted using TMHMM Server v 2.0. Gray bar: transmembrane region, blue: region inside the membrane (cytoplasmic), red: region outside the membrane (exterior or luminal). b Observation of EYFP-expressing transformant and PtPTP::EYFP-expressing transformant through fluorescence microscopy. TL transmission light microscopic images, PAF plastid auto-fluorescence, eYFP enhanced yellow fluorescent protein. Merged: the above images overlaid. Scale bars 5 μm