Fig. 11

Fermentation performance in the presence of acetic acid of bulk-, MSH2-, and HAL9-RHA strains for block 1 in QTL4 on chromosome XV. a Sub-block 1-1, b sub-block 1-3 and c–f individual genes in sub-block 1-2, using in c a longer deletion cassette for MSH2 and in d for HAL9 and using in e a shorter deletion cassette for MSH2 and in f for HAL9. Hemizygous RHA strains containing the MS164 allele (red, 3 or 4 replicates), hemizygous RHA strains containing the 16D allele (blue, 3 or 4 replicates) and diploid hybrid strain MS218 (green). Fermentations were performed in 50 mL YP medium with 40 g/L glucose, supplemented with 12 g/L acetic acid, at pH 4.7, 35 °C and constant stirring at 120 rpm