Fig. 4

Fermentation performance in the presence of acetic acid of the causative block 3-, sub-block 3-1-, and SNF4-RHA strains for QTL1 on chromosome VII. a Causative gene block 3 and b, c gene sub-blocks 3-1 and 3-2 (SNF4). Hemizygous RHA strains containing the MS164 allele (red, 3 or 4 replicates), hemizygous RHA strains containing the 16D allele (blue, 3 or 4 replicates) and diploid hybrid strain MS218 (green). Fermentations were performed in 50 mL YP medium with 40 g/L glucose, supplemented with 12 g/L acetic acid, at pH 4.7, 35 °C and constant stirring at 120 rpm