Fig. 6

Fermentation performance in the presence of acetic acid of TRT2-RHA strains for QTL2 on chromosome XI. a, b Causative block 2, containing only the TRT2 gene, in different concentrations of acetic acid. Hemizygous RHA strains containing the MS164 allele (red, 3 or 4 replicates), hemizygous RHA strains containing the 16D allele (blue, 3 or 4 replicates) and diploid hybrid strain MS218 (green). Fermentations were performed in 50 mL YP medium with 40 g/L glucose, supplemented with 12 g/L (a) or 10 g/L (b) acetic acid, at pH 4.7, 35 °C and constant stirring at 120 rpm