Fig. 9

Fine mapping and linkage analysis of QTL4 on chromosome XV. a SNP variant frequency for selected SNPs in QTL4 and corresponding p values. b QTL4 was divided into eight gene blocks for construction of pairs of hemizygous hybrid strains for evaluation by bulk-RHA. c–e Detailed view of the genes present in the three causative blocks (and their sub-blocks) 1, 3, and 5, respectively. In gene sub-blocks 1-2 (MSH2 and HAL9) (c), 2-1 (IRA2) and 2-2 (REX4) (d) each time two versions of the deletion cassettes for these genes were used: a longer version for deletion of the whole ORF and a shorter version for deletion of most of the ORF while minimizing the risk of affecting the terminator of the adjacent gene. Causative blocks and genes are indicated in red