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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Thermotolerant glycosyl hydrolases-producing Bacillus aerius CMCPS1 and its saccharification efficiency on HCR-laccase (LccH)-pretreated corncob biomass

Fig. 5

Protein profile of cellulase produced by B. aerius CMCPS1. SDS-PAGE and zymogram analysis showing cellulolytic activity. A SDS profile of cellulase produced by CMCPS1 grown on culture medium supplemented with CMC; M—marker; 1—positive check B. licheniformis KBFB3; 2—CMCPS1. B In vitro zymogram of the cellulase activity of the purified enzyme; 1a and 1b—positive check B. licheniformis KBFB3 in two replicates; 2a and 1b—CMCPS1 in two replicates. C Gel diffusion assay of the partially purified cellulase (a) purified cellulase (b) sterile water control. The halo region indicates the cellulase activity of the B. aerius CMCPS1

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