Table 3 Comparison of different pretreatment methods and saccharification efficiency using thermophilic cellulases in CCB
Parameters | Bu et al. [52] | Sukai and Kana [53] | Thangavelu et al. [10] | This study |
|---|---|---|---|---|
Biomass | Corn cob | Corn cob | Corn cob | Corncob |
Source of cellulase | Cellic CTec2 | Celluclast | – | CMCPS1 |
pH | – | – | – | 3–9 |
Temperature °C | – | – | – | 30–90 |
Metal/solvent tolerance | – | – | – | Zn2+; acetone, methanol and isopropanol |
Delignification (%) | 17.82 | 63.61 | 47.44 | 54.1 |
Enzyme loading | 10 FP units g−1 | 12 FP units g−1 and 15 CBH IU g−1 | – | 40 IU g−1; 20 IU g−1 |
Solid loading | 10% | 2.5% | – | 5% |
Saccharification conditions | 47 °C pH 4.8 | 50 °C | – | 50 °C pH 5.0 |
Reducing sugar yield | – | 1.10 g g−1 | – | 1.37 g g−1 (40 IU g−1) 0.99 g g−1 (20 IU g−1) |
Efficiency | Tenfold increase | – | – | 55% |