Table 1 Strains used in this study
From: Growth-uncoupled isoprenoid synthesis in Rhodobacter sphaeroides
Strain | Description | Source of reference |
|---|---|---|
E. coli | ||
S17-1 | Host strain for conjugation, thi pro recA hsdR [RP4–2Tc::Mu–Km::Tn7] TprSmr | Laboratory stock |
R. sphaeroides | ||
Rs265 | Wild-type | Derivative of ATCC35035, Isobionics BV |
Rs265_∆phaC1∆phaC2 | Rs265 with the PHB biosynthetic pathway knocked-out via phaC1 and phaC2 deletion | Isobionics BV |
Rs265_∆phaB | Rs265 with the PHB biosynthetic pathway knocked-out via phaB deletion | [31] |
Rs265-MVA_∆dxr | Rs265 with chromosomally integrated the MVA pathway operon and knocked-out the endogenous MEP pathway via dxr deletion | [16] |
Rs265-MVA_∆dxr∆phaB | Rs265 with chromosomally integrated the MVA pathway operon and knocked-out the endogenous MEP (via dxr deletion) and PHB (via phaB deletion) biosynthetic pathways | This study |