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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Renewable and tuneable bio-LPG blends derived from amino acids

Fig. 4

Effect of the KdcA-dependent pathway promoter on gaseous alkane production in Halomonas TQ10 in the a absence or b presence of valine culture supplementation. Pathway promoter-IHTrKFG462I was present as either a plasmid-borne or chromosomally integrated construct (single site). Cultures (20 mL) were grown in LB medium containing 30 µg/mL kanamycin for 4–6 h (OD600 ~ 0.6–0.8) at 37 °C and 180 rpm. Recombinant protein expression was induced with IPTG (0.1 mM) and culture supplementation with valine (30 mg/mL) after 1 h at 30 °C. Triplicate samples (1 mL) each of 3 biological replicate cultures were sealed into glass vials (4 mL) and incubated at 30 °C for 16–18 h at 200 rpm, illuminated continuously with a blue LED (455 nm or 470 nm). Gaseous hydrocarbon levels were determined by manual headspace injection using an Agilent 490 Micro GC, containing an Al2O3/KCl column. The errors represent one standard deviation of the data

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