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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Enhancement of docosahexaenoic acid production by overexpression of ATP-citrate lyase and acetyl-CoA carboxylase in Schizochytrium sp.

Fig. 1

Development of a β-galactosidase reporter system and selection of strong promoters in Schizochytrium sp. a Construction of reporter plasmid pPICZαA-lacZ. b PCR verification of transformants. +: positive control, reporter plasmid was used as template; −: negative control, WT genomic DNA was used as template; AOX1p-lacZ, ubip-lacZ, EF-1αp-lacZ, ccg1p-lacZ, and TEF-1p-lacZ: the transformants of pPICZαA-AOX1p-lacZ, pPICZαA-ubiquitinp-lacZ, pPICZαA-EF-1αp-lacZ, pPICZαA-ccg1p-lacZ, and pPICZαA-TEF-1p-lacZ. c Phenotypes of the corresponding transformants. Cells were grown for 96 h on GPY plates with 40 μg/mL X-gal. d β-galactosidase enzymatic activities of the transformants. Cells were grown in seed medium for 64 h

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