Fig. 2

Overexpression of ATP-citrate lyase and acetyl-CoA carboxylase in Schizochytrium sp. a Schematic diagram of Schizochytrium sp. ACL and ACC. The proposed enzymatic domains are marked in different colors. b PCR verification of the transformants. +: positive control, the plasmid was used as template; −: negative control, WT genomic DNA was used as template; OACL, OACC, and OACL-ACC: genomic DNA from the transformants of pPICZαA-ACL, pPICZαA-ACC, and pPICZαA-ACL-ACC was used as template