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Table 2 Strains and plasmids used in this study

From: Enhancement of docosahexaenoic acid production by overexpression of ATP-citrate lyase and acetyl-CoA carboxylase in Schizochytrium sp.

Strain or plasmid Description Source or reference
Schizochytrium sp.
 ATCC 20888 Wild-type strain (WT) American Type Culture Collection
 AOX1p-lacZ WT strain carrying pPICZαA-AOX1p-lacZ This study
 ubip-lacZ WT strain carrying pPICZαA-ubiquitinp-lacZ This study
 TEF-1p-lacZ WT strain carrying pPICZαA-TEF-1p –lacZ This study
 EF-1αp-lacZ WT strain carrying pPICZαA-EF-1αp-lacZ This study
 ccg1p-lacZ WT strain carrying pPICZαA-ccg1p-lacZ This study
 OACL ACL overexpression strain This study
 OACC ACC overexpression strain This study
 OACL-ACC ACL and ACC co-overexpression strain This study
E. coli
 JM109 General cloning host for plasmid manipulation Laboratory stock
Plasmids
 pPICZαA Yeast expression vector [42]
 pPICZαA-AOX1p-lacZ lacZ reporter vector using AOX1 promoter and terminator This study
 pPICZαA-ubiquitinp-lacZ lacZ reporter vector using ubiquitin promoter and terminator This study
 pPICZαA-TEF-1p-lacZ lacZ reporter vector using TEF-1 promoter and terminator This study
 pPICZαA-EF-1αp-lacZ lacZ reporter vector using EF- promoter and terminator This study
 pPICZαA-ccg1p-lacZ lacZ reporter vector using ccg1 promoter and terminator This study
 pPICZαA-ACL ACL overexpression vector based on pPICZαA This study
 pPICZαA-ACC ACC overexpression vector based on pPICZαA This study
 pPICZαA-ACL-ACC ACL and ACC co-overexpression vector based on pPICZαA This study