Table 2 Strains and plasmids used in this study
Strain or plasmid | Description | Source or reference |
|---|---|---|
Schizochytrium sp. | ||
ATCC 20888 | Wild-type strain (WT) | American Type Culture Collection |
AOX1p-lacZ | WT strain carrying pPICZαA-AOX1p-lacZ | This study |
ubip-lacZ | WT strain carrying pPICZαA-ubiquitinp-lacZ | This study |
TEF-1p-lacZ | WT strain carrying pPICZαA-TEF-1p –lacZ | This study |
EF-1αp-lacZ | WT strain carrying pPICZαA-EF-1αp-lacZ | This study |
ccg1p-lacZ | WT strain carrying pPICZαA-ccg1p-lacZ | This study |
OACL | ACL overexpression strain | This study |
OACC | ACC overexpression strain | This study |
OACL-ACC | ACL and ACC co-overexpression strain | This study |
E. coli | ||
JM109 | General cloning host for plasmid manipulation | Laboratory stock |
Plasmids | ||
pPICZαA | Yeast expression vector | [42] |
pPICZαA-AOX1p-lacZ | lacZ reporter vector using AOX1 promoter and terminator | This study |
pPICZαA-ubiquitinp-lacZ | lacZ reporter vector using ubiquitin promoter and terminator | This study |
pPICZαA-TEF-1p-lacZ | lacZ reporter vector using TEF-1 promoter and terminator | This study |
pPICZαA-EF-1αp-lacZ | lacZ reporter vector using EF-1α promoter and terminator | This study |
pPICZαA-ccg1p-lacZ | lacZ reporter vector using ccg1 promoter and terminator | This study |
pPICZαA-ACL | ACL overexpression vector based on pPICZαA | This study |
pPICZαA-ACC | ACC overexpression vector based on pPICZαA | This study |
pPICZαA-ACL-ACC | ACL and ACC co-overexpression vector based on pPICZαA | This study |