Fig. 2

KCN11 deletion results in abnormal cell morphology and cell growth. a, b Flagella loss caused by KCN11 mutation under N-depleted condition. Shown are representative cell images after N starvation for 24 h (a) and flagellar loss kinetics during N starvation (b). Data shown are mean ± sem (n = 200). Bar = 5 μm. c Cell volume increase in kcn11 under N-depleted condition. Cells were measured after 24 h at the indicated media. Data shown are mean ± sem (n = 200). N.S., no significant. ***P < 0.0001 (t test). d Cell growth of kcn11 was inhibited following N starvation. Data shown are mean ± sem (n = 200). e, f Loss of chlorophyll is retarded in kcn11 following N starvation. Images of cell cultures grown in N-replete or N-depleted conditions for 24 h (e). Chlorophyll content during N starvation (f). Data shown are mean ± sem (n = 3)