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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Potassium channel KCN11 is required for maintaining cellular osmolarity during nitrogen starvation to control proper cell physiology and TAG accumulation in Chlamydomonas reinhardtii

Fig. 3

KCN11 affects TAG accumulation. a Representative images of cells stained with Nile Red. Red: chlorophyll autofluorescence; Yellow: lipid droplets. Bar = 5 μm. b, c Statistical analysis of the number (b) and size (c) of the lipid droplets. Cells were starved for nitrogen for 2 days. Data shown are mean ± sem (n = 50). ***P < 0.0001 (t test). d Transmission electron microscopy images of WT and kcn11 cells after 2 days of nitrogen depletion. S: starch granule; LD: lipid droplet. Bar = 2 µm. e, f TAG analysis by thin-layer chromatography. Shown are representative image (e) and quantification (f). Cells were starved for nitrogen for 2 days. Data shown are mean ± sem (n = 3). ***P < 0.0001 (t test)

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