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Table 2 The initial concentration of Bacteria and Archaea (number of copies of the 16S RNA from Bacteria or Archaea gene, respectively, per mL of sample), the initial concentration of homoacetogens (number of copies of the FTHFS gene/mL sample), the initial number of hydrogenotrophic methanogens (number of copies of the 16S archaea gene/mL sample*relative abundance of hydrogenotrophic methanogens in the inocula) and the initial number of acetotrophic methanogens (number of copies of the 16S archaea gene/mL sample*relative abundance of acetotrophic methanogens in the inocula) are shown. As well as, the calculated ratio between the amount of Archaea, respectively, to that of the Bacteria (ratio A:B), the amount of hydrogenotrophic methanogens in relation to the amount of homoacetogens (ratio HM:HA) and to the quantity of acetotrophic methanogens (ratio HM:AM). The sum of the relative abundance of Methanobacterium sp., Methanosarcina sp., Methanoculleus sp., Methanobrevibacter sp., Methanosphaera sp., Methanothermobacter sp. and Methanospirillum sp. was used as total relative abundance of hydrogenotrophic methanogens in the inocula, while the sum of the relative abundance of Methanosaeta sp. and Methanosarcina sp. was used as total relative abundance of acetotrophic methanogens

From: Biomethanation processes: new insights on the effect of a high H2 partial pressure on microbial communities

Inoculum Total bacteria Total archaea Ratio A:B Total, HA Total, HM Total, AM Ratio HM:HA Ratio, HM:AM
AnS 1.2 × 1010 5.5 × 108 1:21 9.4 × 108 3.6 × 108 1.5 × 108 1:2.6 1:0.4
GS 1.2 × 1010 5.0 × 109 1:2.5 2.8 × 108 3.8 × 109 2.3 × 108 1:0.1 1:0.1
BM 3.5 × 1010 1.2 × 109 1:29 3.4 × 108 8.3 × 108 3.9 × 108 1:0.41 1:0.5
MFW1 9.8 × 1010 4.5 × 108 1:217 1.9 × 109 3.8 × 108 1.9 × 108 1:4.9 1:0.5
MFW2 4.5 × 1010 2.7 × 108 1:170 6.3 × 108 2.4 × 108 3.3 × 107 1:2.7 1:0.1
FW 8.0 × 1010 9.9 × 107 1:809 1.1 × 108 5.8 × 107 5.0 × 105 1:1.8 1:0.0
AeS 2.9 × 1010 8.6 × 107 1:331 1.1 × 108 2.2 × 107 3.9 × 106 1:4.8 1:0.2
  1. Reference: A: archaea, B: bacteria, HA: homoacetogens, HM: hydrogenotrophic methanogens. Inocula: AnS: anaerobic sludge, GS: granular sludge, BM: livestock manure leachate, MFW1: digestate’s liquid fraction from the 1st stage of the anaerobic digestion of farm waste, digestate’s liquid fraction from the 2nd stage of the anaerobic digestion of farm waste, FW: food waste digestate’s liquid fraction, AeS: aerobic sludge