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Table 1 Results on the synergism between PcAA14B and TtXyn30A on different lignocellulosic substrates

From: A new synergistic relationship between xylan-active LPMO and xylobiohydrolase to tackle recalcitrant xylan

Substrate # Pretreatment conditions Cellulose (% w/w) Acid-insoluble lignin (% w/w) Acid-soluble lignin (% w/w) Hemicellulose (% w/w) TtXyn30A (µM Xylobiose) PcAA14B + TtXyn30A (µM xylobiose) Degree of synergism
1 d. H2O/acetone (50/50%), Air (40% O2), 8.5 bar (175 °C, 2 h) 79.47 8.4 0.5 4.01 1.23 ± 0.00 7.00 ± 0.30 (***) 5.70 ± 0.20
2 d. H2O/acetone (75/25%), Air (40% O2), 40 bar, 175 °C 84.10 11.5 1.0 5.18 0.98 ± 0.06 2.30 ± 0.07 (***) 2.35 ± 0.07
3 Milox 80 °C, 1 h, formic acid 80.30 6.3 1.0 12.35 26.7 ± 1.80 32.3 ± 1.3 (*) 1.21 ± 0.03
4 d. H2O/acetone (25/75%), Air (40% O2), 40 bar, 175 °C 75.36 2.1 1.6 17.93 36.1 ± 1.20 46.1 ± 2.3 (**) 1.28 ± 0.10
5 Milox 80 °C, 1 h, formic acid, 1 bar 75.31 2.1 1.2 19.21 13.9 ± 1.10 17.2 ± 1.1 (*) 1.24 ± 0.04
6 d. H2O/acetone (25/75%), Air, 20 bar 50.22 15.7 3.0 19.71 3.10 ± 0.25 2.20 ± 0.00 (*)
7 Milox 80 °C, 3 h, acetic acid 51.84 4.6 3.0 29.06 20.1 ± 1.00 18.0 ± 0.8 (ns)
  1. The data on the pretreatment conditions and compositional analyses have been transferred by [19]. Error bars represent the standard deviation from three independent experiments. Asterisks (*p < 0.1; **p < 0.01; ***p < 0.001) indicate significance based on Student’s t test.
  2. ns not significant