Table 1 Results on the synergism between PcAA14B and TtXyn30A on different lignocellulosic substrates
Substrate # | Pretreatment conditions | Cellulose (% w/w) | Acid-insoluble lignin (% w/w) | Acid-soluble lignin (% w/w) | Hemicellulose (% w/w) | TtXyn30A (µM Xylobiose) | PcAA14B + TtXyn30A (µM xylobiose) | Degree of synergism |
|---|---|---|---|---|---|---|---|---|
1 | d. H2O/acetone (50/50%), Air (40% O2), 8.5 bar (175 °C, 2 h) | 79.47 | 8.4 | 0.5 | 4.01 | 1.23 ± 0.00 | 7.00 ± 0.30 (***) | 5.70 ± 0.20 |
2 | d. H2O/acetone (75/25%), Air (40% O2), 40 bar, 175 °C | 84.10 | 11.5 | 1.0 | 5.18 | 0.98 ± 0.06 | 2.30 ± 0.07 (***) | 2.35 ± 0.07 |
3 | Milox 80 °C, 1 h, formic acid | 80.30 | 6.3 | 1.0 | 12.35 | 26.7 ± 1.80 | 32.3 ± 1.3 (*) | 1.21 ± 0.03 |
4 | d. H2O/acetone (25/75%), Air (40% O2), 40 bar, 175 °C | 75.36 | 2.1 | 1.6 | 17.93 | 36.1 ± 1.20 | 46.1 ± 2.3 (**) | 1.28 ± 0.10 |
5 | Milox 80 °C, 1 h, formic acid, 1 bar | 75.31 | 2.1 | 1.2 | 19.21 | 13.9 ± 1.10 | 17.2 ± 1.1 (*) | 1.24 ± 0.04 |
6 | d. H2O/acetone (25/75%), Air, 20 bar | 50.22 | 15.7 | 3.0 | 19.71 | 3.10 ± 0.25 | 2.20 ± 0.00 (*) | – |
7 | Milox 80 °C, 3 h, acetic acid | 51.84 | 4.6 | 3.0 | 29.06 | 20.1 ± 1.00 | 18.0 ± 0.8 (ns) | – |