Fig. 1

Gene structure and transcripts at the ace3 locus. a Schematic presentation of ace3 gene annotations at its native locus. Exons are shown as arrows and introns as single lines. In parenthesis are the origins of gene annotations. ▼, Transcriptional start site (TSS); dashed line, non-sense mutation; Cys: cysteine of the Zn₂Cys₆ binuclear domain. b Transcription levels of ace3 exons in strain QM9414 grown on different carbon sources for 24 h, as measured by RNA-seq. Normalized read counts are graphed using 100-bp bins per million mapped reads (BPM). c RT-qPCR analysis of expression levels of ace3 exons, Intron 2, and 5′UTR part of Intron 2. The 5′UTR is located within intron 2 and upstream of the ace3-SC ORF. The transcript expression levels are normalized to the house keeping gene act1, and are shown as relative ratios to the level of exon 1 at early growth phase. The mean ± standard deviation was calculated from three independent experiments