Fig. 4

ace3 deletion. a Schematic representation of the different ace3 variants. Exons are shown as boxes and introns as single lines. The N-terminal and C-terminal amino acid residues are shown. C2 and C4 indicate the presence of 2 and 4 cysteines of the Zn₂Cys₆ binuclear domain within exons, respectively. b SDS-PAGE gel from culture supernatants. An equal volume of culture supernatant was loaded in each lane. c Total secreted protein concentrations as measured by Bradford assays. The mean ± standard deviation was calculated from three biological replicates. d MULac activities measured from the culture supernatants. The mean ± standard deviation was calculated from three biological replicates. Lac: lactose; Glu: glucose