Modification of transcriptional factor ACE3 enhances protein production in Trichoderma reesei in the absence of cellulase gene inducer

Table 1 Total secreted protein titers of T. reesei parental strain T4abc and engineered strains expressing ace3-L driven from different promoters under inducing and non-inducing conditions

Strain ID	Promoter (protein ID¹)	Glu/Sop²	Glu³
T4abc (parental)	N/A	1.00	0.20
LT82	OPT (44278)	1.16	1.07
LT83	dic1(47930)	1.35	1.12
LT85	gut1 (58356)	0.95	1.08
LT86	hxk1 (73665)	0.96	0.73
LT87	pki1 (78439)	1.16	0.98
LT149	rev3 (103041)	0.96	0.76
LT150	Trire2_104295	0.94	0.41
LT151	tkl1 (2211)	1.02	1.01
LT152	bxl1 (121127)	1.00	1.04
LT154	dld1 (5345)	1.00	0.58
LT155	xyn4 (111849)	0.95	0.95
LT156	glr1 (72526)	0.95	0.89
LT157	axe1 (73632)	1.01	0.85
LT158	ace3EL (77513)	1.02	0.29
LT242	Trire2_69944	1.00	0.82
LT246	Trire2_67752	1.00	0.74
LT252	Trire2_3739	1.01	0.87
LT258	Trire2_2499	1.05	0.79

Protein ID¹ is based on JGI QM6a annotation (genome.jgi.doe.gov/Trire2/Trire2.home.html)
Glu/Sop² is an abbreviation of “Glucose/Sophorose”; inducing condition
Glu³ is an abbreviation of “Glucose”; non-inducing condition
Relative protein concentration with the parental strain on Glu/Sop (set as 1) is shown. The numbers are the average from at least three biological replicates

ISSN: 2731-3654