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Table 3 CBP and SSF parameters of the different xylose-consuming S. cerevisiae strains in corn cob liquors

From: Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts

Corn cob liquor

Potential xylose (g/L)

Strain (inoculum)

Glucosei (g/L)

Xylosei (g/L)

Xylosef (g/L)

Ethanolf (g/L)

Xylitolf (g/L)

Acetic acidi (g/L)

Acetic acidf (g/L)

Yxyol/potential xyl (g/g)

Yet/potential sugar (g/g)

Liquor 29XPot (S0 = 3.67 and LSR = 8 g/g)

24.2

ER-X-2P (50 g/L)

0.392 ± 0.003

1.80 ± 0.02

0.418 ± 0.016

6.51 ± 0.26

2.71 ± 0.00

1.13 ± 0.02

1.88 ± 0.02

0.112 ± 0.000

0.247 ± 0.010

CAT-1-X-2P (50 g/L)

0.392 ± 0.003

1.80 ± 0.02

1.77 ± 0.01

3.73 ± 0.37

3.97 ± 0.37

1.13 ± 0.02

1.99 ± 0.60

0.164 ± 0.015

0.142 ± 0.014

CA11-X-2P (50 g/L)

0.392 ± 0.003

1.80 ± 0.02

1.29 ± 0.17

4.80 ± 0.58

2.35 ± 0.14

1.13 ± 0.02

1.66 ± 0.09

0.0971 ± 0.0059

0.182 ± 0.022

Liquor 32XPot (S0 = 3.99 and LSR = 8 g/g)

31.6

ER-X-2P (50 g/L)

0.293 ± 0.003

5.02 ± 0.11

2.11 ± 0.28

8.15 ± 0.54

4.59 ± 0.42

1.81 ± 0.00

2.17 ± 0.20

0.145 ± 0.013

0.240 ± 0.016

ER-X-2P (100 g/L)

0.293 ± 0.003

5.02 ± 0.11

0.873 ± 0.019

11.1 ± 0.2

4.75 ± 0.13

1.81 ± 0.00

2.37 ± 0.01

0.150 ± 0.004

0.328 ± 0.005

Liquor 54XPot (S0 = 3.78 and LSR = 8 g/g)

50.7

ER-X-2P (100 g/L)

0.487 ± 0.010

4.62 ± 0.02

14.0 ± 0.8

4.23 ± 0.04

6.19 ± 0.67

2.53 ± 0.01

3.91 ± 0.34

0.122 ± 0.013

0.0778 ± 0.0008

Whole slurry (liquor 54XPot with 5% solid loading)

50.7 + 6.13

ER-X-2P (100 g/L)

0.487 ± 0.010

4.62 ± 0.02

14.4 ± 1.0

4.20 ± 0.15

6.41 ± 0.21

2.53 ± 0.01

3.72 ± 0.32

0.113 ± 0.004

0.0463 ± 0.0017

Liquor 32XPot (S0 = 3.99 and LSR = 8 g/g) plus 0.017% (v/v) hemicellulase

31.6

ER-2P (100 g/L)

0.466 ± 0.151

5.15 ± 0.19

0.976 ± 0.095

4.65 ± 0.16

0.760 ± 0.022

1.90 ± 0.16

3.38 ± 0.08

0.0240 ± 0.0007

0.137 ± 0.005

ER-X-2P (100 g/L)

0.466 ± 0.151

5.15 ± 0.19

6.55 ± 0.25

6.26 ± 0.06

5.40 ± 0.08

1.90 ± 0.16

3.41 ± 0.19

0.171 ± 0.003

0.184 ± 0.002

Liquor 32XPot (S0 = 3.99 and LSR = 8 g/g) plus 0.050% (v/v) hemicellulase

ER-2P (100 g/L)

0.466 ± 0.151

5.15 ± 0.19

1.02 ± 0.00

6.26 ± 0.10

0.854 ± 0.125

1.90 ± 0.16

3.65 ± 0.22

0.0270 ± 0.0040

0.184 ± 0.003

ER-X-2P (100 g/L)

0.466 ± 0.151

5.15 ± 0.19

8.65 ± 0.02

6.00 ± 0.16

5.01 ± 0.08

1.90 ± 0.16

3.60 ± 0.24

0.159 ± 0.003

0.176 ± 0.005

  1. Assays were performed at 40° C and oxygen-deprived conditions. Data represents the average ± SD from two biological replicates
  2. S0: severity; LSR: liquid-to-solid ratio; Yet/potential sugar: ethanol yield from potential sugars; Yxyol/potential xyl: xylitol yield from potential xylose