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Table 4 Saccharomyces cerevisiae strains and main plasmids used in this study

From: Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts

 

Relevant features

Source

Saccharomyces cerevisiae

 Ethanol Red (ER)

Commercial ethanol yeast

Fermentis, S. I. Lesaffre, Lille

 PE-2

Isolated from a Brazilian first generation bioethanol plant

[14, 49]

 CAT-1

Isolated from a Brazilian first generation bioethanol plant

[14, 49]

 CA11

Isolated from a Brazilian “cachaça” fermentation processes

[13, 50]

 ER-X

ER, pI23-BGL1-kanMX, pI5-XylA-NatMX, pδW-XYN-kanMX

This work

 PE-2-X

PE-2, pI23-BGL1-kanMX, pI5-XylA-NatMX, pδW-XYN-kanMX

This work

 CAT-1-X

CAT-1, pI23-BGL1-kanMX, pI5-XylA-NatMX, pδW-XYN-kanMX

This work

 CA11-X

CA11, pI23-BGL1-kanMX, pI5-XylA-NatMX, pδW-XYN-kanMX

This work

 ER-X-2P

ER-X, pMEC1049 + XI

This work

 CAT-1-X-2P

CAT-1-X, pMEC1049 + XI

This work

 CA11-X-2P

CA11-X, pMEC1049 + XI

This work

 ER-2P

ER, pMEC1049 + XI

This work

Plasmids

 pI23-BGL1-kanMX

SED1p–SED1ss–A. aculeatus BGL1–SAG1a–SAG1t, KanMX marker, I23 integration site

This work

 pI5-XylA-NatMX

SED1p–SED1ss–A. oryzae XYLA–SED1a–SAG1t, NatMX marker, I5 integration site

This work

 pCRE-hyg6

CRE recombinase, hyg6

This work

 pδW-XYN-kanMX

SED1p–SED1ss–T. reesei XYNII–SED1a–SAG1t, KanMX marker, δ sequences integration site

This work

 pMEC1049 + XI

pYPK4-TEF1tp-XR (N272D)-TDH3tp-XYL2-PGI1tp-XKS1-FBA1tp-TAL1-PDC1tp, xylA gene from C. phytofermentans (HXT7p and CYCt), HphMX4

[31]