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Table 2 Single-nucleotide polymorphisms (SNPs) in mutant strains 3.5M and 3.6M compared to wild-type ZM4

From: Development and characterization of acidic-pH-tolerant mutants of Zymomonas mobilis through adaptation and next-generation sequencing-based genome resequencing and RNA-Seq

Locus

SNP

AA change

3.5M

3.6M

Gene

Product

424761

C/T

A67T

99.76

98.86

ZMO0421 (hisC2)

Histidinol-phosphate aminotransferase

711194

G/A

G539D

99.24

99.73

ZMO0712 (ppk)

Polyphosphate kinase

1449594

G/A

P480L

100

99.72

ZMO1432

Efflux pump membrane component

1779278

C/A

T7K

100

99.71

ZMO1733 (oxyR)

Transcriptional regulator OxyR

1306151

C/T

W485*

99.5

–

ZMO1291

Peptidase S10 serine carboxypeptidase

1701191

G/A

L77F

99.08

–

ZMO1651 (ptsP)

Signal transduction protein

173653

T/C

 

100

–

Intergenic region

Between ZMO0183 and ZMO0184

1451222

A/G

 

–

100

Intergenic region

Between ZMO1432 and ZMO1433

  1. The numbers in the columns of 3.5M and 3.6M represent the frequency (%) of the SNP identified in all genome resequencing reads, and “–” indicates the absence of SNP. AA means amino acid. * indicates stop codon
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