Skip to main content
Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Novel xylose transporter Cs4130 expands the sugar uptake repertoire in recombinant Saccharomyces cerevisiae strains at high xylose concentrations

Fig. 2

Subcellular localization of tested transporters in S. cerevisiae cells. a Design of EBY_Xyl1 cells with an integrated XR/XDH/XKS pathway and a multi-copy vector expressing the transporter fused with a codon-optimized eGFP-ymUkG1 (Created with BioRender). b A confocal image confirms the presence of a fluorescence halo in the cell periphery indicating the correct localization of the transporter in the cell membrane. DIC and fluorescence images were taken by a confocal microscope and merged. Scale bars = 1 μm. c Crystallographic and modeled structures of Cs4130 and Gxf1, respectively, embedded in the cytoplasmatic membrane. Cs4130 and Gxf1 show the typical fold of MFS transporters. The 12 transmembrane α-helix in Cs4130 and Gxf1 were assigned based in XylE

Back to article page