|
XylE
|
Cs4130
|
Gxf1
|
DtX (Å)
|
---|
Residue (kcal/mol)
|
---|
Wild-type
|
− 27.4
|
− 28.8
|
− 26.0
|
–
|
Mut1
|
F24A (− 27.6/0.2)
|
F58A (− 27.8/1.0)
|
F58A (24.9/1.1)
|
3.9
|
|
G83A
|
G120A
|
G120A
|
22.0
|
|
R133C
|
R153C
|
R153C
|
11.7
|
|
E153A
|
E173A
|
E173A
|
17.8
|
|
R160A
|
R180A
|
R180A
|
14.3
|
Mut 2
|
Q168A (− 28.3/0.9)
|
Q188A (− 26.4/2.4)
|
Q188A (− 25.5/0.5)
|
2.3
|
Mut 3
|
Q288A (− 26.4/1.0)
|
Q314A (− 28.1/0.7)
|
Q314A (− 24.9/1.1)
|
2.9
|
Mut 4
|
Q289A (− 26.6/0.8)
|
Q315A (− 28.5/0.3)
|
Q315A (− 26.1/0.1)
|
3.0
|
Mut 5
|
N294A (− 28.1/0.7)
|
N320A (− 28.2/0.6)
|
N320A (− 25.2/0.8)
|
2.9
|
Mut 6
|
Y298A (− 26.4/1.0)
|
Y324A (− 28.1/0.7)
|
Y324A (− 25.8/0.2)
|
4.3
|
Mut 7
|
N325A (− 27.4/0)
|
N349A (− 26.9/1.9)
|
N349A (− 25.7/0.3)
|
6.7
|
|
G340A
|
G364A
|
G364A
|
15.8
|
|
R341A
|
R365A
|
R365A
|
14.3
|
Mut 8
|
W392A (− 26.6/0.8)
|
Y428A (− 27.9/0.9)
|
Y431A (− 25.6/0.7)
|
3.1
|
|
E397A
|
E433A
|
E436A
|
14.2
|
|
R404A
|
K440A
|
R443A
|
16.1
|
Mut 9
|
Q415A (− 26.8/0.6)
|
N451A (− 28.0/0.8)
|
N454A (− 26.6/0.6)
|
2.7
|
Mut 10
|
W416A (26.5/0.9)
|
W452A (− 28.5/0.3)
|
W455A (− 25.4/0.6)
|
4.8
|
- Residues close and far from ligand (xylose) are highlighted in italics and underlined, respectively
- The energy of binding (kcal/mol) of mutants and the difference from the wild-type are between parentheses
- Mut mutant