Fig. 1
Identification of Zmo0994 overproduced under stresses in Z. mobilis. a Fermentation profiles of Z. mobilis. Initial cell density was set to 0.5 of optical density at 600 nm (OD600) and cell growth, glucose and ethanol concentrations were monitored during ethanol fermentation. b Overproduction of Zmo0994 at 24 h, when glucose was completely depleted and ethanol concentration was at the maximum. Figure was reprinted and adapted with permission from the reference [69]. c Quantitative RT-PCR analysis for zmo0994 expression in the absence and presence of ethanol in Z. mobilis. When the cell density of Z. mobilis grew to 0.5 of OD600, cells were either treated or non-treated with 6% (v/v) ethanol. Then, the total RNA was isolated from Z. mobilis and was converted into cDNA. Finally, PCR was performed to amplify the partial fragment of zmo0994 (188 bp). As a housekeeping gene, partial fragment of 16 s ribosomal DNA (129 bp) was amplified as shown. The experimental data represent means ± standard deviations from either two or three independent experiments