Fig. 7
E. coli harboring N-terminal truncated zmo0994 exhibited ethanol tolerance. a Plot construction based on hydrophilicity index and percentage of glycine of Zmo0994, hydrophilins from microbes, and LEA proteins from plants. The annotated hydrophilins were acquired from Garay-Arroyo et al. [46] and of LEA proteins from plants were used as a control set (Additional file 17: Note S1), and the hydrophilicity index was determined using webserver-based program available at https://web.expasy.org/protscale [70]. b E. coli harboring signal peptide truncated zmo0994 [E. coli ZMsig(−)] still exhibits ethanol tolerance. For the cell growth test, when the cell density grew to 0.5 of OD600, IPTG and ethanol were added to the culture at final concentrations of 0.1 mM and 4% (v/v), respectively. The experimental data present means ± standard deviations from three independent experiments