Skip to main content
Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Recombinant bacteriophage LysKB317 endolysin mitigates Lactobacillus infection of corn mash fermentations

Fig. 2

SDS-PAGE and recombinant LysKB317 endolysin zymogram assay. Whole cell lysate of overnight induced E. coli strain E. cloni 10G/pRham N-His Kan::LysKB317, soluble fraction from the whole cell lysate (2.5 µg) and purified LysKB317 endolysin (0.25 µg) were run on a 15% SDS–polyacrylamide gel. a Left panel: LabSafe Gel Blue stained gel. b Right panel: Zymogram activity assay. The gel contained bacterial cells of L. fermentum 0605-B44 polymerized within the gel matrix. Following electrophoresis, the gel was washed with deionized water for 1 h, then incubated in 1% Triton X-114, 50 mM Tris, pH 5.5 buffer until zones of clearing were visible (indicated hydrolase activity of LysKB317)

Back to article page