Fig. 3
Spot plate assay of LysKB317 showed exolytic activity against L. fermentum 0315–25. Purified LysKB317 was spot onto an MRS agar plate that contained a soft top 0.7% agar with L. fermentum 0315–25 (OD600 = 0.8; 1 mL). Whole cell lysate expressing LysKB317 (5 µL; E. cloni 10G/pRham N-His kan::LysKB317; Table 1), purified LysA2 (5 µL; negative control; [54]), 20 µg/mL lysozyme (positive control), and 5 µL of MRS broth (negative control) were spotted on to plate and allow to air dry before incubating at 37 °C until zone of clearance can be visualized