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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Recombinant bacteriophage LysKB317 endolysin mitigates Lactobacillus infection of corn mash fermentations

Fig. 4

Activity of LysKB317 endolysin against various strains of bacteria isolated from fuel ethanol fermentation plant. Recombinant LysKB317 was tested for activity against the indicated strains isolated from ethanol fermentation facility using a turbidity reduction assay. In the wells of a 96-well microtiter plate, bacteria were suspended in 100 µL assay buffer (300 mM NaCl, 30% (v/v) glycerol, 21 mM citric acid, 58 mM Na2HPO4, pH5.5; OD600 nm = 2.0), and 100 µL of enzyme reaction (1 µM) added. The change in optical density at 600 nm was measured over a period of 30 min. Data are reported as the mean change in O.D. per minute per µM enzyme (n = 3 independent replicates; error bars indicate standard errors of the mean (SEM))

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