Fig. 5

The endolysin LysKB317 pH stability profile over time under room temperature. Using the turbidity reduction assay, LysKB317 was tested in different pH (4.0–7.5) assay buffer and shown to be active at pH as low as 4.5 for 30 min. to pH 7.5 for 48 h. Time frames of 0.5 h (black bar), 24 h (white bar), and 48 h (gray bar) were arbitrary chosen to measure pH exposure over time. The change in optical density at 600 nm was measured over a period of 30 min at 37 °C. Data are reported as the mean change in O.D. per minute per µM enzyme (n = 3 independent replicates; error bars indicate standard errors of the mean (SEM))