Fig. 6

The endolysin LysKB317 can withstand high temperature of 60 °C up to 72 h. The temperature stability of the endolysin over time was measured using turbidity reduction assay. A temperature gradient ranging from 4 to 95 °C was tested over a period of 0.5 h (black bar), 24 h (white bar), 41 h (gray bar), and 72 h (checker bar). The change in optical density at 600 nm was measured over a period of 30 min. Data are reported as the mean change in O.D. per minute per µM enzyme (n = 3 independent replicates; error bars indicate standard errors of the mean (SEM))