Fig. 7

The endolysin LysKB317 is stable under 30% ethanol over 72 h at room temperature. A turbidity reduction assay was performed under various concentration of ethanol (0%, 5%, 10%, 20% and 30%) imitating conditions of LysKB317 would be exposed to in a fermentation tank at a bioethanol refinery plant. A time period of 0 h (black bar), 27 h (white bar), 48 h (dark gray bar), and 72 h (light gray bar) was used to test the stability of LysKB317 under ethanol exposure. The change in optical density at 600 nm was measured over a period of 30 min. Data are reported as the mean change in O.D. per minute per µM enzyme (n = 3 independent replicates; error bars indicate standard errors of the mean (SEM))