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Fig. 8 | Biotechnology for Biofuels

Fig. 8

From: Recombinant bacteriophage LysKB317 endolysin mitigates Lactobacillus infection of corn mash fermentations

Fig. 8

Small-scaled corn mash fermentation treatment with exogenously added LysKB317 reduced L. fermentum population. Cultures of S. cerevisiae grown on corn mash feedstock alone and exogenously added LysKB317 without L. fermentum challenge (served as negative controls not shown (below detection limit 3-log CFU/mL). Fermented corn mash challenged with 106 CFU/mL of L. fermentum 0315–25 (gray triangle) served as positive control for contamination. Treatment applied to challenged fermentation corn mash (Black circle) showed significant bacterial contamination reduction over 72 h closely matching level of unchallenged fermented corn mash at 72 h. Data are reported in log CFU/mL growth of L. fermentum. Bacterial CFU counts were enumerated and log transformed every 24 h for 72 h using MRS agar plants. Error bars indicate standard errors of the mean (SEM); three independent replicates. *P < 0.05 based on one-way ANOVA

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