Skip to main content

Table 1 Bacteria and yeast strains used in this study

From: Recombinant bacteriophage LysKB317 endolysin mitigates Lactobacillus infection of corn mash fermentations

Bacteria and yeast

Relevant genotype/phenotypea, b

Reference or sourcec

Escherichia coli

 E. cloni 10G

mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL (StrR) nupG λ− tonA

Lucigen Co

 E. cloni 10G/pUC57::LysKB317

AmpR, containing LysKB317 gene

GenScript, This study

 E. cloni 10G/pRham N-His Kan::LysKB317

KanR, containing LysKB317 gene

This study

 BL21(DE3)

F− ompT hsdSB (rB-mB-) gal dcm (DE3)

Invitrogen

 BL21(DE3)/pET21a::LysA

AmpR, containing LysA gene

[4]

Acetobacter pomorum

 150316 F1.18

Wildtype

This study

Enterococcus faecium

 B-41204

Wildtype

NRRL

 1410-7.24

Wildtype

NRRL

Lactobacillus amylovorus

 0315-7B

Wildtype

[51]

 150316 F2.23

Wildtype

This Study

Lactobacillus brevis

 0605-48

Wildtype

[4]

 1410-6.6

Wildtype

This study

Lactobacillus casei

 091009 7.25

Wildtype

This study

 1410-5.41

Wildtype

This study

Lactobacillus delbrueckii

 B-1924

Wildtype

NRRL

 B-4525

Wildtype

NRRL

 B-763

Wildtype

NRRL

Lactobacillus fermentum

 B-1840

Wildtype

NRRL

 B-1932

Wildtype

NRRL

 0315-1

Wildtype

[51, 52]

 0315-25

Wildtype

[4, 51]

 0605-B44

Wildtype

[4]

 091009–8.21

Wildtype

This study

 1101-7.13

Wildtype

This study

 1410-1.1

Wildtype

This study

 1502-8.10

Wildtype

This study

Lactobacillus johnsonii

 1412-7.32

Wildtype

This study

Lactobacillus mucosae

 0713-2

Wildtype

[53]

 0315-2B

Wildtype

This study

Lactobacillus plantarum

 1101 7.25

Wildtype

[52]

 1410-5.32

Wildtype

This study

Lactobacillus rossiae

 1410-5.34

Wildtype

This study

Pediococcus acidilactici

 B-14958

Wildtype

NRRL

Pediococcus pentosaceus

 B-14620

Wildtype

NRRL

Staphylococcus lugdunensis

 1502-8.20

Wildtype

 

Streptococcus agalactiae

 B-1815

Wildtype

NRRL

Streptococcus uberis

  

USDA

Weissella confusa

 0216-2

Wildtype

This study

Saccharomyces cerevisiae

 Y-2034

 

NRRL

  1. aAmpR, Ampicillin resistant; KanR, kanamycin resistant
  2. bWildtype microbial strains were isolated from a Midwestern dry-grind fuel ethanol plant and selected from a previous screen [15]
  3. cUSDA-ARS Culture Collection, Peoria, IL (also known as the NRRL Collection)