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Table 2 Treatment model for bacterial load and fermentation products of experimentally infected ethanol fermentations treated with exogenously added LysKB317 endolysin

From: Recombinant bacteriophage LysKB317 endolysin mitigates Lactobacillus infection of corn mash fermentations

Treatment L. fermentum 0315–25     
LysKB317 (nM) Log (CFU/mL) Ethanol (g/L) Glucose (g/L) Lactic (g/L) Acetic (g/L)
Control  < 3.0 117.4 ± 7.9 4.5 ± 2.6 15.2 ± 0.4 0.9 ± 0.2
0 8.4 ± 0.2 97.0 ± 7.5 38.9 ± 2.7 19.8 ± 0.5 3.6 ± 0.5
1 8.6 ± 0.1 94.6 ± 1.6 37.2 ± 0.6 19.9 ± 0.6 4.3 ± 0.8
10 8.5 ± 0.1 95.8 ± 7.4 26.1 ± 4.8 19.7 ± 0.1 2.7 ± 0.1
100 6.5 ± 0.2 113.1 ± 3.9 6.9 ± 4.1 16.3 ± 0.7 1.4 ± 0.3
1,000 6.4 ± 0.1 115.1 ± 13 2.1 ± 0.1 16.6 ± 0.1 1.3 ± 0.2
10,000 4.1 ± 1.4 118.3 ± 4.5 0.7 ± 0.3 15.4 ± 0.1 1.0 ± 0.3
  1. Cultures of S. cerevisiae grown on corn mash feedstock were challenged with 106 CFU/mL of L. fermentum 0315–25 [27], and treated with the indicated concentration of recombinant LysKB317 endolysin. The control culture was not challenged with L. fermentum 0315–25. After 72 h incubation, viable L. fermentum was determined by enumeration on MRS agar plates, and the fermentation broth was analyzed by HPLC for the following fermentation products: ethanol, residual glucose, lactic acid, and acetic acid