Skip to main content

Table 2 Treatment model for bacterial load and fermentation products of experimentally infected ethanol fermentations treated with exogenously added LysKB317 endolysin

From: Recombinant bacteriophage LysKB317 endolysin mitigates Lactobacillus infection of corn mash fermentations

Treatment

L. fermentum 0315–25

    

LysKB317 (nM)

Log (CFU/mL)

Ethanol (g/L)

Glucose (g/L)

Lactic (g/L)

Acetic (g/L)

Control

 < 3.0

117.4 ± 7.9

4.5 ± 2.6

15.2 ± 0.4

0.9 ± 0.2

0

8.4 ± 0.2

97.0 ± 7.5

38.9 ± 2.7

19.8 ± 0.5

3.6 ± 0.5

1

8.6 ± 0.1

94.6 ± 1.6

37.2 ± 0.6

19.9 ± 0.6

4.3 ± 0.8

10

8.5 ± 0.1

95.8 ± 7.4

26.1 ± 4.8

19.7 ± 0.1

2.7 ± 0.1

100

6.5 ± 0.2

113.1 ± 3.9

6.9 ± 4.1

16.3 ± 0.7

1.4 ± 0.3

1,000

6.4 ± 0.1

115.1 ± 13

2.1 ± 0.1

16.6 ± 0.1

1.3 ± 0.2

10,000

4.1 ± 1.4

118.3 ± 4.5

0.7 ± 0.3

15.4 ± 0.1

1.0 ± 0.3

  1. Cultures of S. cerevisiae grown on corn mash feedstock were challenged with 106 CFU/mL of L. fermentum 0315–25 [27], and treated with the indicated concentration of recombinant LysKB317 endolysin. The control culture was not challenged with L. fermentum 0315–25. After 72 h incubation, viable L. fermentum was determined by enumeration on MRS agar plates, and the fermentation broth was analyzed by HPLC for the following fermentation products: ethanol, residual glucose, lactic acid, and acetic acid