|
Treatment
|
L. fermentum 0315–25
| | | | |
|---|
|
LysKB317 (nM)
|
Log (CFU/mL)
|
Ethanol (g/L)
|
Glucose (g/L)
|
Lactic (g/L)
|
Acetic (g/L)
|
|
Control
|
< 3.0
|
117.4 ± 7.9
|
4.5 ± 2.6
|
15.2 ± 0.4
|
0.9 ± 0.2
|
|
0
|
8.4 ± 0.2
|
97.0 ± 7.5
|
38.9 ± 2.7
|
19.8 ± 0.5
|
3.6 ± 0.5
|
|
1
|
8.6 ± 0.1
|
94.6 ± 1.6
|
37.2 ± 0.6
|
19.9 ± 0.6
|
4.3 ± 0.8
|
|
10
|
8.5 ± 0.1
|
95.8 ± 7.4
|
26.1 ± 4.8
|
19.7 ± 0.1
|
2.7 ± 0.1
|
|
100
|
6.5 ± 0.2
|
113.1 ± 3.9
|
6.9 ± 4.1
|
16.3 ± 0.7
|
1.4 ± 0.3
|
|
1,000
|
6.4 ± 0.1
|
115.1 ± 13
|
2.1 ± 0.1
|
16.6 ± 0.1
|
1.3 ± 0.2
|
|
10,000
|
4.1 ± 1.4
|
118.3 ± 4.5
|
0.7 ± 0.3
|
15.4 ± 0.1
|
1.0 ± 0.3
|
- Cultures of S. cerevisiae grown on corn mash feedstock were challenged with 106 CFU/mL of L. fermentum 0315–25 [27], and treated with the indicated concentration of recombinant LysKB317 endolysin. The control culture was not challenged with L. fermentum 0315–25. After 72 h incubation, viable L. fermentum was determined by enumeration on MRS agar plates, and the fermentation broth was analyzed by HPLC for the following fermentation products: ethanol, residual glucose, lactic acid, and acetic acid
