Table 5 Primers used in this study
Primer name | Sequence (5′–3′) | Purpose | Reference |
|---|---|---|---|
Sau_F | CATCATCACCACCATCACGCACTTTACGTAGTTGACGTT | Amplification of LysKB317 for cloning | This study |
Sau_R | GTGGCGGCCGCTCTATTATTTAAAGGTTCCGAATGCTTC | ||
pRham_F | GCTTTTTAGACTGGTCGTAGGGAG | Verify gene insert | Lucigen Co |
pETite_R | CTCAAGACCCGTTTAGAGGC |