Fig. 2

Transport studies with radiolabeled sugars. a Amount of lactose uptaken by yeast strains expressing N. crassa \(\upbeta\)-glucosidase and different transporters during the 20-min assay with 5 mM lactose. The dots present the values obtained for the biological replicates (n = 4) and the bars present their average. b Kinetics of cellobiose (top) or lactose (bottom) transport by yeast strains expressing CRT1-RUT-C30 or CDT-1. Error bars present standard deviation between 2–3 independent experiments. c Inhibition of cellobiose uptake by CDT-1 or CRT1 by different sugars in yeast. Cellobiose concentration in the reaction was 5 mM and inhibitor concentration was 25 mM. Error bars present standard deviation from 2 replicates from 2 individual experiments (n = 4)