Fig. 1

Ascospore production and antibiotic susceptibility of T. aurantiacus: a Germination rates were assessed from spores of fungal cultures incubated at 50 °C for 2 days and then 45 °C for 1–6 days. At the indicated time, spores were scraped from 3 replicate plates for each day; germination was detected via randomized counts of spore suspensions. b The total amount of produced spores was calculated with a hemocytometer. Growth tests of T. aurantiacus on different selection markers: c hygromycin B, nourseothricin and geneticin (PDA medium), d phleomycin (Vogel’s minimal medium) and e glufosinate ammonium (Vogel’s minimal medium). f T. aurantiacus is able to grow on acetamide (Vogel’s minimal medium with no nitrogen added, ammonium nitrate and acetamide from left to right). Two replicate plates were used for all assays. Note that all antibiotics or acetamide were separately sterile filtered and added to the media after autoclaving. Two biological replicates were used for each test